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KMID : 0377619700190030225
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Korean Jungang Medical Journal
1970 Volume.19 No. 3 p.225 ~ p.233
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Effect of Chloramphenicol and Cortisone on the Immunization of Rabbits and the Bactericidal Action of the Sera
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Abstract
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Recently many works have been reported concerning the effect of chloramphenicol on the protein synthesis. The findings, however, are not always agreeable each other; some asserting that the synthesis of protein is inhibited by the presence of chloramphenicol while others reporting the opposite role of the antibiotics. Cortisone is a hormone also known as an agent having complicated influences on the protein synthesis.
Employing rabbits as experimental animals and Salmonella typhi H901 as a test organism, anexperiment was undertaken to see whether chloramphenicol and cortisone have any effect on the antibody formation and the bactericidal action of the immune sera with the following results.
1. Primary, secondary, and tertiary immune responses to the heat-killed Salmonella antigens of the rabbits treated with chloramphenicol or cortisone were not significantly different each other or from the non treated control. Dosages of chloramphenicol and cortisone in each injection were 100mg/kg or 200 mg/kg and 10 mg/kg or 20 mgikg, respectively. Number of injections were from once to 15 for chloramphenicol and from twice to 15 for cortisone. The first injection was done 10 min. before first antigen inoculation and the remaining at 12 hour intervals following the first in both cases.
2. In spite of the indistinct agglutination titer between immune sera, the bactericidal action of the sera showed a marked difference between the chloramphenicol and cortisone-treated rabbits serum and the non-treated control. The heavier dosages of chloramphenicol or cortisone were given, the lower was the bactericidal action of sera.
3. When serum was diluted with sterile saline, the difference of bactericidal activities between the sera disappeared gradually following the degree of dilution, finally reaching the level of saline solution.
4. The author has tried to find a clue in the alternation of complement components or in.
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